Protein Modification Reagents
Worthington Biochemical Corporation COLLANGENAS
Chromatographically purified. <50 caseinase units per milligram. Supplied as a lyophilized powder.
Worthington Biochemical Corporation NEUTRAL PROTEASE 10MGNEUTRAL
Chromatographically purified. A lyophilized powder.
Advanced Biomatrix Collagenase, Lyophilized Powder, 50 mg
This Collagenase product is provided as a lyophilized, sterile powder in a 50 mg package size. It has been tested specifically and shown to effectively hydrolyze collagen and dissociate tissues. Collagenase consists of a blend of enzymes including collagenase, caseinase, clostripain and trypsin designed to effectively hydrolyze collagen and dissociate tissues. Various collagenase products have been evaluated specifically for use with Advanced BioMatrix’ s collagen products. This product is offered to improve the viability and functionality of isolated cells from the digested collagen gels. After reconstitution, the product is ready-to-use.
Genscript Corporation TEV Protease, His
Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the tags from fusion proteins. The optimal temperature for cleavage is 30°C; also it can be used at temperature as low as 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
Avanti Polar Lipids DSPE-PEG(2000) Maleimide Powder | 474922-22-0 | 25mg
DSPE-PEG(2000) Maleimide Powder | Purity: 99% | 474922-22-0 | 25mg
Creative Pegworks PSB-451 4-Arm PEG-MAL, MW 10k, 10 grams
4-Arm PEG-MAL is a multiarmed PEG derivative with maleimide at each terminal of the four arms connected to one pentaerythritol core. Maleimide selectively reacts with free thiol, SH, sulfhydryl, or mercapto group via Michael addition to form a stable thioether bond. PEG-MAL useful for site specific protein and peptide modification. Hydroquinone MEHQ at ~ 200 ppm may be present as inhibitors to prevent self polymerization and partial crosslinking. MEHQ may be removed per customer request.
Worthington Biochemical Corporation COLLAGENASE TYPE II FILTERED
Collagenase, Type 2 (Code: CLS-2), which is filtered through a 0.22 micron membrane and lyophilized in vials.
TRIBIOSCIENCE INC DTT Solution (1M), DNase / RNase Free, 50 ML
Tribioscience’s Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. By reducing the disulfide bonds in a protein sample, the protein can be more effectively fragmented and analyzed. The concentration is 1M.
Sigma Aldrich Fine Chemicals Biosciences Trypsin from bovine pancreas | 9002-07-7 | MFCD00082094 | 50 mg
Trypsin from bovine pancreas | Mol Wt: 23.8 kDa | 9002-07-7 | MFCD00082094 | 50 mg